Current Research

1. Ion Channel Signalling and Physiology

We focus on the regulation and functional modulation of ion channels, which are essential for maintaining cellular physiology and are implicated in various pathological conditions. Our goal is to understand how these ion channels interact with intracellular signalling molecules that play critical roles in cell division and stress responses. These interactions are being investigated using a combination of computational modelling and experimental approaches, which include protein-protein interaction assays, immunofluorescence, and electrophysiology. Through this study, we aim to uncover novel molecular mechanisms that contribute to disease progression and provide insights for the development of targeted therapeutic strategies.

Immunofluoresence staining for co-localization studies

Transmission Electron Micrographs of membrane micro-domains

2. Mechanobiology of Cancer Invasion

In this project we are interested to explore the biomechanical aspects of breast cancer cells and aim to establish if biomechanical sensors can be novel targets in cancer therapy. We investigate key structural proteins that may modulate membrane biomechanics to regulate cell shape and motility, thereby influencing cancer cell migration and invasiveness. We use a combination of techniques such as protein-protein interaction assays, immunofluorescence and scanning ion conductance microscopy (SICM) coupled with pressure jet.

We aim to elucidate how differential protein expression shapes the biomechanical and cytoskeletal properties underlying distinct invasive behaviours. Using Scanning Ion Conductance Microscopy (SICM), coupled with a pressure jet, an advanced biomechanical imaging technique, we explore the role of these structural proteins in the physical regulation of invasiveness. This research aims to uncover how structural protein-mediated mechanical cues drive metastasis and may identify potential mechanobiological markers and therapeutic targets for tumour progression.

3. Zebrafish Disease Models

An innovative zebrafish xenograft model is simultaneously being developed and validated to facilitate real-time in vivo monitoring of cancer metastasis and tumor invasion. Unlike traditional rodent models, the zebrafish system offers high cost-effectiveness and reduced resource utilization. The development of this model will serve as a powerful tool for studying cancer cell dynamics, validating therapeutic targets, and screening anti-cancer compounds with high efficiency and lower ethical concerns compared to mammalian systems.

Representative image of control and chemotherapuetic drug treated xenografts.

Pipeline of ZebraTrack method for measuring swimming behaviour of zebrafish

4. Impact of "Contaminants of Emerging Concern (CECs)" on health

In this project we are interested to evaluate the impact of "Contaminants of Emerging Concern (CECs)" on human health using zebrafish as a vertebrate animal model. We evaluate the impact on various aspects of cellular health including cardiotoxicity, neurotoxicity and reproductive toxicity. In particular we are interested in exploring whether exposure to these contaminants can cause mental health disorders. This includes exploring the temporal dynamics of anxiety behaviour, recovery, and the underlying molecular mechanisms in zebrafish exposed to emerging pollutants. To quantify anxiety behaviour in zebrafish, we have developed an open-source method (in collaboration) that serves as a cost-effective alternative to commercially available softwares. This research will advance our understanding of the impact of contaminants on health including mental health and also help in identifying novel molecular targets for therapeutics.

Report abuse